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p raf 1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology p raf 1
    P Raf 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 741 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+raf+1/Raf-1/pm41814365-84-2-29
    Average 94 stars, based on 741 article reviews
    p raf 1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Dexamethasone Inhibits Repair of Human Airway Epithelial Cells Mediated by Glucocorticoid-Induced Leucine Zipper (GILZ)
    Article Snippet: .. After blocking, the membrane was incubated with anti-GILZ antibody (1∶100 dilution, Santa Cruz Bio, CA, USA)and sequentially with anti-Raf-1, p-Raf-1, Mek1/2, p-Mek1/2, Erk1/2, p-Erk1/2 antibodies (1∶1000 dilution, Cell Signaling, Danvers, MA, USA) overnight at 4°C. .. Afterwards, horseradish peroxidase-conjugated anti-mouse/rabbit secondary antibodies (MultiSciences, Hangzhou, China) were used.

    Membrane:

    Article Title: Dexamethasone Inhibits Repair of Human Airway Epithelial Cells Mediated by Glucocorticoid-Induced Leucine Zipper (GILZ)
    Article Snippet: .. After blocking, the membrane was incubated with anti-GILZ antibody (1∶100 dilution, Santa Cruz Bio, CA, USA)and sequentially with anti-Raf-1, p-Raf-1, Mek1/2, p-Mek1/2, Erk1/2, p-Erk1/2 antibodies (1∶1000 dilution, Cell Signaling, Danvers, MA, USA) overnight at 4°C. .. Afterwards, horseradish peroxidase-conjugated anti-mouse/rabbit secondary antibodies (MultiSciences, Hangzhou, China) were used.

    Incubation:

    Article Title: Dexamethasone Inhibits Repair of Human Airway Epithelial Cells Mediated by Glucocorticoid-Induced Leucine Zipper (GILZ)
    Article Snippet: .. After blocking, the membrane was incubated with anti-GILZ antibody (1∶100 dilution, Santa Cruz Bio, CA, USA)and sequentially with anti-Raf-1, p-Raf-1, Mek1/2, p-Mek1/2, Erk1/2, p-Erk1/2 antibodies (1∶1000 dilution, Cell Signaling, Danvers, MA, USA) overnight at 4°C. .. Afterwards, horseradish peroxidase-conjugated anti-mouse/rabbit secondary antibodies (MultiSciences, Hangzhou, China) were used.

    other:

    Article Title: EPHA2-Ephrin-B1 cis-interaction drives an oncogenic reverse signaling, leading to the recurrence of oral cancer.
    Article Snippet: The antibodies, p-Raf-1, p38 MAPK (MAPK 1/3), TUBB, TUBB2A, TUBA1C, TUBB4B, ERO1L, TUBB3, NDRG1, GAL3, FSCN, GAL1, DSG3, ALDH1A1, Ephrin-B1, EPHA2, EPHB2, mouse IgG and β-Actin were purchased from Santa Cruz Biotechnology, Inc., Dallas, Texas, USA.

    Article Title: Ameliorative and Synergic Effects of Derma-H, a New Herbal Formula, on Allergic Contact Dermatitis
    Article Snippet: 2,4-dinitrochlorobenzene (DNCB) and Dexamethasone was purchased from Sigma Aldrich (St.Louis, USA).

    Transfection:

    Article Title: Apigenin promotes melanogenesis and melanosome transport through the c-KIT/Raf-1/MAPK/CREB pathway in HEMCs
    Article Snippet: Antibodies against MITF (ab140606, 1:1000), Tyrosinase (ab180753, 1:1000), TRP-1 (ab235447, 1:1000), TRP-2 (ab221144, 1:1000), and p-MSK1 (ab32190, 1:2000) were purchased from Abcam (Cambridge, UK). .. Anti-Myosin Va (sc-365986, 1:200), KIF5b (sc-133184, 1:200), Cdc42 (sc-8401, 1:200), Rab27a (sc-74586, 1:200), p-c-KIT (sc-365504, 1:200), c-KIT (sc-13508, 1:200), p-Raf-1 (sc-271929, 1:200), Raf-1 (sc-7267, 1:200), p-p38 (sc-166182, 1:200), p38 (sc-398546, 1:200), p-ERK (sc-7383, 1:200), ERK (sc-514302, 1:200), p-RSK (sc-377526, 1:200), RSK (sc-74575, 1:200), p-MEK (sc-81503, 1:200), MEK (sc-13159, 1:200), MSK1 (sc-518173, 1:200), p-PKA (sc-293036, 1:200), PKA (sc-390548, 1:200), p-CREB (sc-81486, 1:200), CREB (sc-240, 1:200), CRTC1 (sc-271333, 1:200), c-KIT siRNA (sc-29225), Rab 27a siRNA (sc-41834) and Cdc42 siRNA (sc-29256), siRNA Transfection Reagent (sc-29528), siRNA Transfection Medium (sc-36868) were obtained from Santa Cruz Biotechnology (Dallas, United States). .. The AC inhibitor 2′,5′-dideoxyadenosine (DDA, HY-135878), MC1R antagonist Nonapeptide-1 (N-1A, HY-P0097A), c-KIT receptor inhibitor ISCK03 (HY-101443), and RSK inhibitor BI-D1870 (HY-10510) were purchased from MedChemExpress (New Jersey, United States).



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    RNF115 facilitates the ubiquitinated degradation of CDK10 and then activates the <t>Raf-1</t> pathway in thyroid carcinoma (THCA) cells. A Subcellular localization of RNF115 and CDK10 by immunofluorescent staining (scale bar = 20 µm). B The interaction between RNF115 and CDK10 by CoIP assay. C CDK10 ubiquitination was measured by western blotting. D Western blotting shows the expression of RNF115, CDK10, and Raf-1 pathway-related proteins in TPC-1 and SNU-790 cells. Cells were transfected with Ad-RNF115 and/or CDK10. *p < 0.05 and **p < 0.01 vs. Ad-NC + Vector; ##p < 0.01 vs. Ad-RNF115 + Vector
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    Image Search Results


    RNF115 facilitates the ubiquitinated degradation of CDK10 and then activates the Raf-1 pathway in thyroid carcinoma (THCA) cells. A Subcellular localization of RNF115 and CDK10 by immunofluorescent staining (scale bar = 20 µm). B The interaction between RNF115 and CDK10 by CoIP assay. C CDK10 ubiquitination was measured by western blotting. D Western blotting shows the expression of RNF115, CDK10, and Raf-1 pathway-related proteins in TPC-1 and SNU-790 cells. Cells were transfected with Ad-RNF115 and/or CDK10. *p < 0.05 and **p < 0.01 vs. Ad-NC + Vector; ##p < 0.01 vs. Ad-RNF115 + Vector

    Journal: Cell Biology and Toxicology

    Article Title: RNF115 aggravates tumor progression through regulation of CDK10 degradation in thyroid carcinoma

    doi: 10.1007/s10565-024-09845-w

    Figure Lengend Snippet: RNF115 facilitates the ubiquitinated degradation of CDK10 and then activates the Raf-1 pathway in thyroid carcinoma (THCA) cells. A Subcellular localization of RNF115 and CDK10 by immunofluorescent staining (scale bar = 20 µm). B The interaction between RNF115 and CDK10 by CoIP assay. C CDK10 ubiquitination was measured by western blotting. D Western blotting shows the expression of RNF115, CDK10, and Raf-1 pathway-related proteins in TPC-1 and SNU-790 cells. Cells were transfected with Ad-RNF115 and/or CDK10. *p < 0.05 and **p < 0.01 vs. Ad-NC + Vector; ##p < 0.01 vs. Ad-RNF115 + Vector

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    Techniques: Staining, Co-Immunoprecipitation Assay, Western Blot, Expressing, Transfection, Plasmid Preparation